动物造模 z-bio 2021-01-28 424

　　Objective: To investigate the effects of Lycium barbarum polysaccharides on the growth and metastasis of breast cancer using mice with Lycium barbarum polysaccharides.

　　 Method: Expand the research team to identify transgenic spontaneous breast cancer MMTV-PyMT mice. The positive female mice were randomly divided into LBP group and control group, with 8 mice in each group. Mice were administered at 8 weeks of age. The LBP group was treated with LBP (50 mg/kg, intraperitoneal injection), and the control group was treated with saline. The tumor volume was measured every 2 days. Four weeks later, the patient was executed after dislocation. Buan solution observes the number of metastatic nodules on the lung surface, fixes the tumor tissue, and uses implanted immunohistochemical methods to detect tumor cell proliferation and blood vessel density.

　　Result: The tumor incidence in MMTV-PyMT positive mice was 100%. Compared with the control group, the tumor weight of the LBP group (4.208±0.4463 g) was significantly lighter than that of the control group (6.477±0.3724 g). , And the tumor volume is also obvious. Less than the control group, the number of lung surface nodules in the LBP group (12±1.155) was significantly lower than that in the control group (20±2.745). Immunohistochemistry results. The staining showed that the number of cell proliferation and microvessel density in the tumor tissue of the LBP group were significantly lower than that of a few control groups.

　　 Conclusion: Lycium barbarum polysaccharide can inhibit the growth and metastasis of breast cancer in MMTV-PyMT mice, and inhibit the proliferation and angiogenesis of tumor cells. MMTV-PyMT mice can be used as a model of breast cancer anticancer drug lung metastasis.