动物模型,前列腺炎 z-bo 2020-07-31 746

　　[Modeling mechanism] Chronic non-bacterial prostatitis can be caused by mycoplasma, chlamydia and autoimmune diseases. The clinical manifestations of chronic bacterial prostatitis are very similar, but not specific. The main symptoms are urgency, dysuria and urine. Local injection of chemicals through the prostate, such as perineal infinity, pain and swelling, can cause chemical inflammation. Alternatively, biological agents are used to stimulate model animals to trigger an immune response and induce immune inflammation in prostate tissue.

　　[Modeling method]

　　1. A traditional surgical kit suitable for rats and C57BL/6 mice, sterile micropipettes, anesthetics and corresponding chemicals, such as carrageenan, xiaozhiling, formaldehyde, croton oil, glycerin, 2 % Agar. Or Freund's complete adjuvant (CFA) DTP vaccine.

　　2. Under anesthesia and aseptic conditions, the male rat makes an incision in the midline of the lower abdomen to expose the prostate, and according to the needs of the experiment, micropipette appropriate amount of inflammatory agent into the prostate. Alternatively, mice were injected intraperitoneally with 0.5 mg of 3 mg/ml CFA, then injected subcutaneously with 1 mg of purified prostate antigen protein, and then injected intraperitoneally with 0.1 ml of DPT vaccine, which resulted in prostatitis within 8 weeks.

　　3. Visual observation of sample collection and observation indicators for the status of the lateral lobe prostate disease; weigh the wet prostate wet weight and calculate the relative weight; calculate the total number of white blood cells and lecithin body density in the prostatic fluid; prostate pathological biopsy is based on inflammatory cells in the prostate infiltration. Record the degree of fibroblast proliferation, the size of the glandular cavity in the prostate, and the amount of secretion in the glandular cavity. The degree of inflammatory cell infiltration and fibroblast proliferation in the prostatic stroma was scored on a 4-point scale, and the size of the prostate gland cavity and glandular secretion were scored on a 3-point scale. The differences in histopathological scores between different treatment groups were compared and statistically analyzed. Determine the effect of the drug. [Model Features] The prostate has increased a considerable weight, and microscopic examination shows that the histology of the prostate has been damaged to varying degrees. Heterogeneous tissue proliferation or atrophy, duct dilation or injury, partial destruction of basement membrane, increased or decreased secretion, rat prostate stromal edema and a large number of lymphocytes, monocytes, neutrophils, etc. When inflammatory cells infiltrate, the secretion in the glandular cavity is reduced or eliminated, a large number of inflammatory cells are present, and glandular epithelial cells are shed. [Model Evaluation and Application] The cause of this animal model is acute chemical inflammation, with rapid and severe inflammation and pathological reactions, and even extensive necrosis of prostate tissue. These symptoms are inconsistent with clinical manifestations, pathological manifestations are very different, and lack pathological specificity. Histopathological biopsy and electron microscopy of the prostate specimens of experimental animals confirmed chronic inflammatory lesions similar to human chronic nonbacterial prostatitis. At the same time, experimental mouse glands such as thymus, submandibular gland and spleen were also observed. , Did not show pathological changes similar to the prostate, indicating that the experimental method has excellent specificity. The model can be used to study the cause of chronic nonbacterial prostatitis, drug screening and efficacy evaluation.

　　In addition to the non-bacterial prostatitis model caused by the injection of chemicals or biological agents, other methods include: (1) castration and estrogen-induced rat prostatitis; (2) neonatal mouse thymic autoimmune prostatitis model; ③Genetic natural prostatitis model in old rats; ④Pelvic floor injection of lactic acid, etc.