动物造模,自身免疫性脑脊髓炎 z-bio 2021-10-27 741

　　OBJECTIVE: To observe the effects of different concentrations of yellow ical glycosides on experimental autoimmune encephalomyelitis (EAE) mouse models and to explore its preliminary mechanism.

　　Method: Establish experimental autoimmune myelitis mouse model. On the third day after immunization, high and low doses of bacitracin were administered once a day for 20 days. Mouse activity nerve function score, TUNEL staining to detect cell apoptosis in spinal cord tissue, ATP level detection kit to detect spinal cord tissue ATP content, Bax, Bcl-2, cleavedcas-9, and cleavedcas-3 protein expression Western blotting levels.

　　Results: Bicin can improve the neurological function of experimental autoimmune encephalomyelitis mice and delay its onset time; after Vitin intervention, tunnel staining results show the number of apoptotic cells in the spinal cord. Western blot results show significant Bcl- Increasing I found that Bax, cleavedcas-9 and cleavedcas-3 protein expression were 2 protein expressions and significantly decreased, which indicated that ATP levels were decreased and decreased.

　　Conclusion: Visine can inhibit apoptosis by inhibiting endogenous mitochondrial cell apoptosis, protect mitochondria and improve the nerve function of mice with experimental autoimmune encephalomyelitis. This provides an experimental theoretical basis for disease prevention.