动物造模,促红细胞生成素 z-bio 2021-01-29 336

　　Objective: To investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of brain-derived neurotrophic factor (BDNF) in various parts of the brain tissue of aging rats.

　　Methods: 40 2-month-old male SD rats were randomly divided into 4 groups: negative control group (N), D-galactose group (D), EPO intervention group (E), positive control group (P), Every 10 groups. Immunohistochemical staining was used to observe the changes of BDNF expression in different parts of the brain tissue of D-galactose-aging rats after the intervention of exogenous rhEPO.

　　Results: Comparing rats in different groups, we found significant differences in the expression of BDNF in the same area: The number of BDNF positive cells in the hippocampus CA1, CA3, DG and frontal cortex of rats in group D was significantly lower than that in group N (P\u003c0. 05) The number of BDNF positive cells in hippocampus CA1, CA3, DG and cerebral cortex motor area of rats in E and P groups after rhEPO intervention was compared with the same part, and the difference was statistically significant. Both groups D and N increased (P\u003c0.05), but there was no difference between group E and group P. The comparison of BDNF-positive cells in different parts of the same group of rats showed that the number of BDNF-positive cells in different parts of the same group of rats was significantly different (P\u003c0.05). The number of positive cells in the frontal lobe is highest in the cortex, followed by hippocampal CA3 area, hippocampal DG area and hippocampal CA1 area.

　　Conclusion: rhEPO is universal in enhancing the expression of BDNF in rat neurons, which indicates that rhEPO can protect the nervous system from aging by enhancing endogenous BDNF.