动物造模,小鼠肾缺血再灌注损伤 z-bio 2022-04-28 532

　　Objective: To study ultrasound radiation to promote the adhesion of target microbubbles and improve the sensitivity and feasibility of renal ischemia-reperfusion injury detection.

　　Method: Establish a mouse renal ischemia-reperfusion model and a normal control group. 1, 3, 6, 12 and 24 hours after reperfusion are divided into IR1h, IR3h, IR6h, IR12h and IR24h groups. According to whether there is ultrasound radiation, each group is randomly divided into one-click targeted microbubble (MBICAM) group and targeted microbubble + ultrasound radiation group (MBICAM + USRF) group.

　　 Results: The control kidneys of the MBICAM and MBICAM + USRF groups did not increase significantly, and the value of renal acoustic intensity (VI) was not significantly different (6.47±0.42 vs. 6.45±0.62, P = 0.923). Renal IR injury showed enhanced imaging in different time frames, and the imaging intensity gradually increased over time. In each time range of ischemia-reperfusion, the kidney VI level of the MBICAM + USRF group was significantly higher than that of the MBICAM group, and there was a significant difference between the two (P = 0.000). There was no significant difference between the IR12h and IR24hVI values (P = 1.000), and there were also significant differences between the other groups (P\u003c0.05).

　　 Conclusion: The application of ultrasound radiation combined with anti-cell adhesion molecule 1 targeting microbubble monoclonal antibody can improve the detection sensitivity of renal ischemia-reperfusion injury and microvascular inflammation in mice, or can be used for early evaluation of related things.