动物造模 z-bio 2021-01-29 676

　　Objective: To microinject K252a into the hippocampus to study its influence on rat behavior and classic depression-like indicators, and to establish a new type of depression animal model.

　　Method: SD rats were randomly divided into 5 groups: blank control group, sham operation group, chronic stress depression model group, hippocampal microinjection K252a group, hippocampus microinjection K252a and chronic stress group. Field experiment, sugar water consumption experiment, Morris water maze experiment is used to measure the influence of rat behavior, enzyme combined immunosorbent measurement is used to detect serum monoamine transmitter content, radiation changes, immunoassay is used to detect rat plasma The change. CRH and ACTH. Observe the changes of CORT content, BDNF, CREB, ERK1/2 and BCL-2 protein expression in the hippocampus of western blotting rats.

　　Result: Compared with the blank control group, the activity level, sugar consumption, learning and memory ability (P\u003c0.05 or P\u003c0.01) and HPA axis function of rats in the CUMS group increased significantly. Decreasing serum monoamine transmitter levels (P \u003cu003c0.01) will down-regulate the expression of BDNF, CREB, ERK1/2 and BCL-2 (P \u003cu003c0.01). (U003c0.05), but the above indicators were not statistically different in the DMSO group; compared with DMSO, the activity, sugar consumption, learning and memory abilities of the K252a and K252a + CUMS groups were significantly reduced (P). P \u003cu\→ u003c0.05), reduce the serum monoamine transmitter level (P \u003cu\→ u003c0.01 or P \u003cu003c0.05\→), and significantly improve the HPA axis function (P \u003cu\→ u003c0 .05). Hippocampus BDNF, CREB, ERK1/2 and BCL-2 were significantly reduced (P\u003c0.01 or P\u003c0.05) (P\u003c0.01 or P\u003c0.05). There were no significant differences in the above indicators between CUMS, K252a and K252a + CUMS groups. Compared with the K252a group, there was no significant difference in the above indicators in the K252a + CUMS group.

　　 Conclusion: From the different perspectives of behavior, hematology and molecular biology, this model is a classic chronic stress depression in terms of surface validity, structural validity and functional validity, and is very similar to the model. This can provide another research method.