动物造模,PC12细胞凋亡 z-bio 2021-01-29 454

　　Objective: To explore the protective effect of nimodipine on the apoptosis of PC12 cells induced by hydrogen peroxide (H2O2).

　　Method: The PC12 cells were randomly divided into normal group, model group (200μmol/LH2O2) and low concentration, medium concentration and high concentration nimodipine group (1, 10, 100μmol/L nimodipine + 200μmol/LH2O2). The MTT method was used to detect the cell survival rate of each group, and the apoptosis of each group, aspartate proteolytic enzyme (caspase 3), caspase 9 and superoxide dismutase (SOD) activities, C Dialdehyde is stained with hexist. Content, Western blot analysis of B lymphocyte tumor 2 (Bcl-2), Bcl-2 related X protein (Bax) and p53 protein.

　　Result: Compared with the normal group, the model group has decreased cell viability, increased cell apoptosis, increased caspase 3 and caspase 9, SOD activity decreased, MDA content decreased, Bcl-2 expression decreased, Bax and p53 expression increased. Compared with the model group, nimodipine increased cell viability, decreased cell apoptosis, decreased caspase 3 and caspase 9 activities, increased SOD activity, and low, medium and high concentrations of nimodipine statistically increased. This is significant (P\u003c0.01). The content of MDA has increased. The expression of Bcl-2 protein increased while the expression of Bax and p53 decreased. The difference is statistically significant (P\u003c0.01).

　　 Conclusion: Nimodipine can inhibit the apoptosis of PC12 cells induced by H2O2 by regulating the expression of apoptosis-related proteins.