动物造模 z-bio 2021-02-01 356

　　Objective: To study the effect of E. coli prokaryotic expression and purification of proprosin protein on improving cardiac function.

　　Method: Obtain the asprosin coding sequence from GenBank, optimize the codon according to the codon priority of E. coli, synthesize the entire gene, connect it to the expression vector, and perform IPTG-induced expression and purification. A mouse model of cardiac dysfunction was established by ligating and relaxing the left anterior descending artery. Thirty mice were randomly divided into three groups: sham operation group (sham), cardiac dysfunction group (MI/R) and cardiac dysfunction group (MI/R +Asp). Echocardiography examines left ventricular function, assesses the degree of cardiac dysfunction, and examines the effect of asprosin on the improvement of cardiac function.

　　Result: After prokaryotic expression and purification, the purity of the target protein is more than 95%, and the endotoxin content is lower than u003c0.1EU/μg protein, which is very suitable for cell and animal research. The cardiac dysfunction model has been successfully established. Compared with the simple injury group, after exogenous administration of recombinant asprosin protein, the cardiac function of mice was significantly improved (P\u003c0.05).

　　 Conclusion: Asprosin protein can reduce damage to heart function and improve heart function.