动物造模 z-bio 2021-02-01 288

　　Objective: To study the proliferation of umbilical cord mesenchymal stem cells (UC-MSC) in bovine mammary epithelial cells (BMEC) under serum-free co-culture conditions.

　　 Method: Choose the fastest growing UC-MSC and BMEC, and use two co-culture methods of direct contact and indirect contact as the experimental group. Contact directly based on UC-MSC. Each BMEC is 2:1, mixed 1:1, 1:2, 1:3, 1:4, 1:5, 1:10 concentration gradient and co-cultivation. Indirect contact is to extract the supernatant of UC-MSC as a resuspension to adapt to the environment. BMEC. The control group is a simple culture group of UC-MSC and BMEC, and the blank control is negative. The growth changes of each group of cells were 0, 4, 8, 12, 24, 36, 48, 60 hours, and 72 hours. Use the CCK-8 method. The cell proliferation in each group was detected.

　　 Results: At 48 hours, the A value of the conditioned medium BMEC group was significantly higher than the A value of the control group (P\u003c0.05), but the A value of the 1:2 concentration group reached the peak. Very important. It is higher than the control group (P\u003c0.01), and is significantly higher in other concentration groups and the conditioned medium BMEC group (P\u003c0.05).

　　 Conclusion: Under serum-free conditions, the co-culture of UC-MSC and BMEC can promote the growth of BMEC. The growth effect of direct contact is better than that of indirect contact. The best ratio is 1:2, and the best time is 48 hours.