动物造模 z-bio 2021-02-01 424

　　Objective: To investigate the effect of glucose supplementation on AMPK activation of exercise myocardium by measuring the changes of AMP-activated protein kinase (AMPK) activity and glycogen content in rats after acute exercise.

　　Method: Rats undergo acute endurance exercise and supplemented with different doses of glucose at different times before and after exercise. Western blot method was used to detect the dynamic changes of rat myocardial AMPK activity, and the Anthlone method was used to detect rat myocardial glycogen content.

　　Result: Exercise significantly increased the AMPK activity in the rat myocardium, and maintained a high level of 1 hour after the acute exercise, but the AMPK activity in the rat myocardium was significantly increased after exercise. Neither exercise nor low-dose sugar supplementation caused significant changes in the glycogen content of rats. 24 hours after exercise, only high-dose sugar supplements can significantly increase glycogen content.

　　 Conclusion: (1) Acute exercise can increase the activity of AMPK in the rat myocardium, and glucose supplementation can significantly inhibit the activation of AMPK during and after exercise.