动物造模,2型糖尿病 z-bio 2021-02-01 320

　　OBJECTIVE: To compare the ulcer healing characteristics of C57BL/6J mice induced by C57BL/6J-db/db and STZ. These mice have spontaneous mutations in commonly used genes in type 2 diabetic mice with stable diabetes, providing a basis for establishing mouse ulcer models Relevant animal research is being carried out.

　　Method: Establish a diabetic mouse ulcer model, and calculate the dynamic healing rate and healing time of the mouse wound on 0, 3, 5, 7, 10 and 14 days. Tissues were collected from the 7th and 14th days, and Masson staining was used for HE staining to observe the pathological changes of the wound, immunohistochemistry (CD31 and PCNA); the gene expression of healing related factors collagen-Ⅲα and α-SMA; fluorescence quantification Determination.

　　Results: Compared with STZ-induced mice, the wound healing time of genetic mutation db/db mice was significantly delayed from (16.6±0.8) d to (20.2±1.3) d (P\u003c0.001); db/db group Compared with STZ, the growth of granulation tissue in this group was slower, the length of new epithelium was insufficient, collagen deposition was blocked and wound healing was insufficient. The expression of CD31 and PCNA in the db/db group was significantly insufficient (P\u003c0.01). The genes in the db/db group were up-regulated on the 7th and 14th day. The multiple of the number was significantly lower than the STZ group.

　　Conclusion: Both diabetic mice delayed wound healing, but compared with STZ-induced mice, db/db mice with genetic mutations are more suitable for diabetes due to delayed wound healing and difficulty in healing.