【Animal Modeling】-The influence of air sac on the results of abdominal wall withdrawal reflex experiment

  Purpose: To select suitable materials to prepare balloons for abdominal wall retraction reflex experiments.

  Method: Use digital display calipers and X-ray images to measure different airbag diameters at different pressure values, and use three pain thresholds as the standard to test whether the airbags cause obvious visceral pain pressure in rats in different air value.

  Results: With the increase of pressure, the diameter of the round latex balloon changes uniformly and gently, which can meet the pressure range commonly used in experiments (20-80mmHg). The threshold for causing visceral pain in rats is almost harmful. Stimulus value. It causes irreversible damage to the intestines of rats.

  Conclusion: For the abdominal wall contraction reflex experiment, it is ideal to use a round latex balloon.