动物造模,miR-424,肺癌细胞株 z-bio 2021-02-01 263

　　Objective: To investigate the effect of miR-424 on the migration and infiltration of non-small cell lung cancer A549 cells.

　　Method: RT-detection of miR-424 expression and lipid in lung cancer cells NCI-H460, NCI-H1975, NCI-H446, A549, NCI-H1299, NCI-H157 and MRC-PCR of human embryonic lung fibroblasts. Method 5 Lipofectamine TM2000 transfers miR-424 inhibitor and miR-424NC to A549 cells. After 48 hours, the RT-PCR method detected the expression of miR-424, the CCK-8 method detected cell viability, the cell migration detected by the scratch test, and the cell infiltration detected by the transwell test. Westernblot detects the expression of matrix metalloproteinase 2 (MMP2), MMP9, transforming growth factor-β1 (TGF-β1) and p-Smad3.

　　Results: miR-424CI-H460, NCI-H1975, NCI-H446, A549, NCI-H1299, NCI-H157 [(1.78±0.13), (1.69±0.10), (1.89±0.18)) cells in lung cancer), (2.88±0.27), (2.52±0.20), (2.49±0.23)] The expression level is miR- (P\u003c0.01) at the expression level of human embryonic lung fibroblasts MRC-5 (0.58±0.05). Significantly higher than 424. Compared with the miR-424NC group, the expression of miR-424 in the miR-424 inhibitor group was significantly reduced (P\u003c0.01), cell viability was reduced (P\u003c0.01), cell migration and The invasion ability was reduced (P\u003c0.01), while the expression levels of MMP2, MMP9, TGF-β1 and p-Smad3 were significantly down-regulated (P\u003c0.01).

　　Conclusion: The down-regulation of miR-424 expression may inhibit the migration and infiltration of A549 cells by inhibiting the TGF-β1/Smad3 signaling pathway.