动物造模,大鼠心梗后血管新生 z-bio 2021-11-01 262

　　OBJECTIVE: To study the effect of a single local injection of small dose of simvastatin into the bone on angiogenesis and cardiac function after myocardial infarction in rats.

　　Method: Wistar rats were randomly divided into sham operation group, myocardial infarction model group and intraosseous simvastatin group (n=12). The left anterior descending coronary artery was ligated to establish a rat myocardial infarction model. Twenty-four hours later, the experimental group was given a single injection of simvastatin 0.5 mg into the left tibia. After 4 weeks, the left ventricular function was evaluated by small animal echocardiography, and the myocardial infarction area was calculated by triphenyltetrazolium chloride (TTC) staining. Fluorescence staining detects local angiogenesis.

　　Results: Echocardiographic results showed that the left ventricular systolic function was significantly decreased after 4 weeks of myocardial infarction, and intraosseous injection of simvastatin did not significantly improve the left ventricular function after myocardial infarction in rats; TTC staining revealed that the intraosseous injection of simvastatin group myocardium The infarct size did not decrease significantly; immunofluorescence staining showed that the myocardial blood vessel density did not increase significantly in the intraosseous simvastatin group.

　　Conclusion: A single intraosseous injection of low-dose simvastatin (0.5 mg) in rats 24 hours after myocardial infarction did not significantly improve the area of myocardial infarction, angiogenesis and cardiac function.