动物造模 z-bio 2021-02-01 293

　　Objective: To explore the effect of PM2.5 on the physiology and related biochemical stress effects of rat uterine tissue.

　　 Method: 30 SD female rats were randomly divided into 3 different PM2.5 exposure groups (saline control group, 1.5 mg/kg PM2.5 low-dose group and 37.5 mg/kg PM2.5 high-dose group). After 10 days of PM2.5 exposure, the female mice were sacrificed, and the pathological changes of the uterine tissue were observed by HE staining, and the contents of SOD, GSH, MDA and LDH in the uterine tissue were detected.

　　Result: Compared with the control group, the uterine tissue of female mice in the PM2.5 exposure group was abnormal, the endometrial epithelial cells became thinner, and the arrangement was disordered; the lamina propria stromal cells and blood vessels were reduced. The test results of oxidative stress indicators showed that the contents of MDA and LDH in the high-dose group were (6.53±1.24) nmol/mg prot and (265.62±24.65) U/g prot, which were significantly higher than those in the control group (P<0.05); The contents of SOD and GSH in the group and low-dose group were significantly lower than those in the control group (P<0.05).

　　 Conclusion: PM2.5 can damage the morphology of rat uterus and induce uterine-related oxidative stress.