动物造模,免疫抑制 z-bio 2021-02-01 351

　　Objective: To study the changes of metabolic pathways during the immunosuppressive effect of cyclophosphamide on mice by metabolomics methods.

　　 Method: Use flow cytometry to understand changes in immune cells, and build a partial least squares model with multiple results to make decisions. Screen the potential biomarkers of different importance in the projection (VIP) parameters of the partial least squares model, determine the difference of biotransformants through statistical analysis, and quickly identify and analyze the software system of small molecule compounds with the help of metabolomics. Through database search and calculation, nine different metabolites were finally identified, and three different metabolic pathways were analyzed through the results of pathway enrichment.

　　Result: Cyclophosphamide is the normal biosynthesis of unsaturated fatty acids in the human body, mitochondrial fatty acid metabolism, glycerophospholipid metabolism, steroid hormone biosynthesis, arachidonic acid metabolism, fatty acid metabolism, pyrimidine biosynthesis and other metabolism, which influence the pathway. It has obvious effects.

　　 Conclusion: The most important metabolic pathways affected by cyclophosphamide in the normal and experimental groups are arachidonic acid metabolism, glycerophospholipid metabolism and pyrimidine metabolism.