动物造模,膀胱癌大鼠 z-bio 2021-02-01 326

　　Objective: To analyze the chemical intervention and mechanism of curcumin in a rat model of bladder cancer induced by N-methylnitrosourea (MNU).

　　 Method: 100 SD rats were randomly divided into 4 groups, namely the control group (10), the model group (10), the intervention group (40) and the treatment group (40). The control group has the same time. Injecting physiological saline into the bladder, and injecting bladder MNU into the other three groups induced the formation of a bladder cancer model in SD rats (injecting a 1 mg/mL MNU solution into the bladder). Respectively for 2, 4, 6, 8 weeks, 2 mg each time, once every 2 weeks, 4 times in total), distilled water was injected into the model group, and the intervention group was injected into the rats that induced bladder cancer. Curcumin solution (400μmol )/L) When MNU is injected into the bladder. That is, the bladder was perfused at 1, 3, 5, 7 and 9 weeks, and the rats were euthanized at the 10th week. In the treatment group, after inducing the rat bladder cancer model, the bladder was perfused with curcumin solution (400μmol/L). Bladder perfusion lasted 14, 16 and 18 weeks. The rats were sacrificed at the 19th week. Stain the obtained bladder tissue. Hematoxylin-Eosin (HE) is used to observe physiological changes; TUNEL end labeling is used to determine apoptosis in tumor tissues; Western blot is used to detect the expression of apoptosis-related proteins.

　　Result: The incidence of bladder cancer in the model group was 90% (9/10) at the 10th week, while the incidence of rat bladder cancer in the intervention group at the 10th week was 12.5% (5/40). Yes. The incidence of bladder cancer in the treatment group was 92.5% (37/40) at 10 weeks. There is a significant difference in the incidence of bladder cancer between the intervention group and the model group (P\u003c0.05), and curcumin has a greater impact on MUN-induced bladder cancer. Rats have obvious chemical intervention effects. When curcumin was administered to the treatment group after the formation of bladder cancer, the incidence of bladder cancer was 78.4% (30/37) at 19 weeks. Compared with the 10th week before treatment, curcumin has been shown to be effective for bladder cancer. It has a therapeutic effect and can slow the progression of bladder cancer. TUNEL experiment confirmed that curcumin can significantly promote the apoptosis of bladder cancer cells and inhibit the growth of bladder cancer cells. Western blot results showed that curcumin inhibited NF-κB activation and effectively down-regulated the expression of NF-κB-regulated gene products.

　　 Conclusion: Curcumin has a significant chemical intervention effect on the rat model of bladder cancer induced by MNU. Its mechanism inhibits the activation of NF-κB, and the gene products regulated by NF-κB are effective. The expression of related proteins in the bladder cancer culture medium may inhibit its growth, induce cell apoptosis, conduct anti-cancer chemical interventions and cause the recurrence of bladder cancer.