动物造模 z-bio 2021-02-01 379

　　OBJECTIVE: To investigate the effects of cognitive development and dopamine (DA) and dopamine (3,4-dihydroxyphenylacetic acid, DOPAC) levels in hippocampus of 80-day-old pups born from pregnant rats for fear of injury, and explore the effects of the two Correlation.

　　Methods: The bystander electric shock method was used to establish a pregnant rat model of fear of kidney injury. Morris water maze experiment was performed on 80-day-old pups to observe their cognitive development; brain dialysis was collected from the right hippocampus by a stereotaxic device, and the application was efficient Liquid chromatography-electron capture detection (HPLC-ECD) method was used to determine the content of DA and DOPAC in brain dialysis fluid of offspring.

　　Results: Compared with the control group, the average escape latency of the 80-day-old model group increased, the swimming speed slowed down, the 20% stay time in the marginal area increased, and the number of steps decreased. The difference was significant (P<0.05); 80 days The levels of DA and DOPAC in the extracellular fluid of the hippocampal tissues of the young model group decreased at each perfusion time point, and the difference was significant (P <0.05); the DA and DOPAC levels in the hippocampus of the 80-day-old young mice were compared with the average There is a positive correlation between the escape latency and the number of cross-stations, and a negative correlation with the stay time in the 20% marginal area, and the correlation is significant (P<0.05); DOPAC is positively correlated with its average escape latency and the number of cross-stations, and the correlation is significant (P< 0.05).

　　Conclusion: Fear of injury to pregnant rats can affect the spatial learning and memory ability of their 80-day-old offspring, and reduce the content of DA and DOPAC in the hippocampus. The level of cognitive development is closely related to the decrease in DA and DOPAC content in the hippocampus.