全脑缺血再灌注损伤 z-bio 2021-02-02 280

　　OBJECTIVE: To investigate the protective effect and mechanism of cysteinyl leukotriene receptor antagonists (Prukast, HAMI 3379) on global cerebral ischemia-reperfusion injury in Mongolian gerbils.

　　Methods: The bilateral common carotid arteries were ligated for 10 minutes and reperfusion for 24 hours to establish a global cerebral ischemia-reperfusion injury model in Mongolian gerbils. They were randomly divided into sham operation group, model group, Plukast, and HAMI 3379 group. Group of 20 animals were given intraperitoneal injection 3 days before operation, once a day, 30 minutes before operation. Observe the neurological symptoms and function of 24h after reperfusion; observe the neurons in the cortex and hippocampus by Nissl staining; detect the expression of autophagy-related proteins beclin-1 and LC3 in the cortex and hippocampus by immunoblotting; observe the autophagosomes in the hippocampus by electron microscope . Results Compared with the model group, Plukast and HAMI 3379 group can improve the score of neurological symptoms, reduce nerve function damage, reduce the damage and loss of cortex and hippocampus neurons, and reduce the expression of autophagy-related proteins beclin-1 and LC3 and the hippocampus The number of autophagosomes in the region.

　　Conclusion: Cysteinyl leukotriene receptor antagonists can reduce the cerebral ischemia-reperfusion injury of Mongolian gerbils by down-regulating autophagy in the cerebral cortex and hippocampus.