动物造模,牛子宫内膜上皮细胞 z-bio 2021-10-12 266

　　Objective: To study the induced differential expression of integrin αvβ3 gene in bovine uterine epithelial cells cultured in vitro, and provide a reference for exploring bovine uterine receptor marker protein?

　　Method: Can estrogen change the induced expression of integrin αvβ3 in bovine endometrial epithelial cells, using RT-PCR to analyze different concentrations? Progesterone and estrogen? The synergy of progesterone?

　　Result: The single addition of 10-7mg/mL progesterone resulted in the highest expression of integrin αvβ3. The expression levels of αv and β3 in the 10-7 mg/mL group were significantly higher than those in the control group (P0.05)?

　　 Conclusion: When progesterone is added alone and estrogen and progesterone are used in combination, the mRNA expression of integrin αvβ3 increases. This factor also promotes bovine endometrial epithelial cells, while adding estrogen alone has the opposite effect. Therefore, the integrin αvβ3 can be used as a potential reference for bovine endometrial epithelial uterine resistance during the "implantation window".