动物造模,小鼠肺部炎症 z-bio 2022-01-10 333

　　Purpose: What is the TNF-α in mice infected with Mycoplasma pneumoniae (MP)? What are the levels of serum INF-γ and MPN372 in lung tissue? P1? To study the effect of Qingzao Jiufei Decoction and its decomposition agent on the expression of AQP5. The molecular mechanism of MP infection?

　　 Method: randomly place 144 SPF BABL/c mice in the normal group (group A)? Model group (group B)? QJD group (C group)? Separated into QJD decomposition agent. Group I (group D) QJD degradant group II (group E) and azithromycin group (group F), each with 24 mice? Except for group A, the other 5 groups of mice were treated with MP infection model. Model drugs were administered after the forced feeding therapy was modeled, and samples were taken on the 3rd, 7, 10th, and 14th days after the modelling. Observe pathological sections of mouse lung tissue under a microscope to observe the degree of pathological inflammation. Score the mouse lung tissue and calculate the mouse lung index and dryness. Comparison; MPN372 using qPCR method? Enzyme-linked immunosorbent assay (ELISA) was used to determine the P1 gene content and the changes in serum TNF-. One in each group of mice? INF-γ level; Western blotting to detect the expression of AQP5 protein?

　　Result: After MP infection, we can see under the microscope that the alveolar septum thickens and the bronchial wall thickens. Then, a large number of inflammatory cells infiltrate, their lung index increases, and the dry-to-wet ratio decreases. TNF-α? The content of INF-γ showed an upward trend and reached a peak on the 7th day. On the 14th day, the expression of AQP5 protein tended to decrease and gradually increased. Compared with group B, from day 7 onwards, group C is MPN372? We can down-regulate the expression levels of P1 and TNF-α, and up-regulate The. INF-γ? AQP5 expression level; the main effect of group D is MPN372? P1? It down-regulates the expression of TNF-α, the main effect of group E is INF-γ? Will the expression of AQP5 be upregulated?

　　 Conclusion: QJD can regulate lung inflammation after MP infection, and reduce the production of MP toxin MPN372 and the expression of P1 adhesion protein INF-γ.