动物造模 z-bio 2021-10-11 293

　　Purpose: To use panax notoginseng saponins (PNS) and its monomer Rg1 to induce hypoxic glucose and hypoxic cell injury model to reduce the tight junctions of vascular endothelium caused by hypoxia? Study the protective effect of Rb1.

　　Method: After inoculating HUVEC cells into the transwell cell compartment and fusing the cells, an experimental group was established: a normal control group? Model group? PNS10, 20, 40 mg/L group and Rb113.52 mg/L, Rg112.44 mg/L group received drug intervention during hypoxia and glucose deficiency for 6 hours and 24 hours after reoxygenation. HUVEC detected Changes in endothelial cell resistance and endothelial permeability. Confocal laser microscope and immunofluorescence were used to detect the changes of ZO-1βclaudin-5 protein expression in HUVEC cells.

　　Result: Compared with the normal control group, after 6 hours of hypoxia and 24 hours of reoxygenation, the tight junction resistance between HUVEC cells and endothelial cells was significantly reduced, and the cell permeability was significantly increased (P\u003c0.05). PNS20 and 40 mg/L groups can significantly inhibit the decrease of tight junction resistance and the increase of HUVEC cell permeability in the model group (P\u003c0.05). Ginsenoside Rb1 may significantly increase the tight junction resistance of the endothelium and reduce the permeability of HUVEC cells after 24 hours of reoxygenation at 6h of OGD (P\u003c0.05). Immunofluorescence detection results showed that the ZO-1 and claudin-5 proteins of HUVEC cells in the normal control group were mainly distributed around the cell membrane and arranged continuously. This is a typical phenomenon of endothelial cells and shows the arrangement of paving stones. After 6 hours of hypoxia and 24 hours of reoxygenation, the cell-terminal tight junction protein of the model group was significantly reduced, the loop structure was destroyed or disappeared, and the nuclear tight junction protein tended to increase. PNS20, 40 mg/L and Rb113.52 mg/L showed local restoration of tight junctions between cells and showed paving stone-like structures.

　　 Conclusion: PNS has a protective effect on the damage of tight junctions between vascular endothelial cells caused by ischemia.