动物造模,大鼠脊髓损伤 z-bio 2021-02-02 303

　　OBJECTIVE: To investigate the effect of artificially recombined botulinum toxin type A heavy chain (BoNT/A heavy chain) on the expression of local growth-related proteins in rats with spinal cord injury, and provide evidence for elucidating its mechanism.

　　Methods: Establish a rat model of unilateral lumbar spinal cord injury, and deliver BoNT/A heavy chain locally and intrathecally; perform two-dimensional electrophoresis on local bone marrow tissue at different times after treatment, and perform overall protein expression; growth-related proteins Under the influence of BoNT/A heavy chain, the expression and distribution of GAP-43 (GAP-43) and upper cervical ganglion protein 10 (SCG10) were detected by Western blot and immunofluorescence.

　　Results: (1) Unilateral lumbar spine injury model animals showed obvious unilateral motor and sensory dysfunction; (2) BoNT/heavy chain administration after spinal cord injury may affect the local protein expression profile of the injury. Possible: BoNT/heavy chain can cause The local protein spot group is damaged, and the expression is reversed or increased based on the change, especially the MW located at 35-45 kDa and 18-25 kDa. Protein spots with an isoelectric point in the range of 5-7 are clear; (3) Western blot and immunofluorescence staining results show that the BoNT/A heavy chain can promote the expression of p-GAP43 and SCG10 (P\u003c0.05), p- GAP43 and SCG10 were mainly positive in the cells around the injury, and all cytoplasm and processes were positive.

　　Conclusion: After BoNT/SCI, administration of heavy chain may promote the expression of selective growth-related proteins p-GAP43 and SCG10 after SCI.