动物造模,维生素D,自发性糖尿病大鼠 z-bio 2021-02-02 302

　　Objective: To investigate the effect of vitamin D deficiency on the etiology of spontaneous diabetic rats.

　　Method: According to body weight, male Zucker control mice and model mice (ZDF) aged 5-6 weeks are randomly divided into 4 groups according to their body weight, namely normal control group (ZL)? Divided into a control group lacking VD (ZL + VD.Def). Model group (ZDF) and VD have no model group (ZDF + VD.Def). Rats in each group were fed to 12 weeks of age. During the feeding process, monitor body weight, food intake, water consumption, urine output, urine glucose and blood glucose levels, and perform oral glucose tolerance tests at week 11. Age; HE staining is used to observe the morphological structure of rat pancreatic islets.

　　Results: The weight of rats in the VD-deficient model group was significantly higher than that of the model group; the increase in fluid intake and urine output was earlier than that of the model group; the blood glucose level of the VD-deficient model group was significantly faster than that of the model group. The blood glucose level of the VD-deficient model group at all stages was significantly higher than that of the model group, about 2.0 times that of the 12-week-old model group. Compared with the model group, VD patients lacking the model group have impaired glucose tolerance. The damage to islet cells is more serious.

　　Conclusion: VD deficiency can accelerate diabetes in ZDF rats and aggravate its development.