动物造模,小鼠造血干细胞 z-bio 2021-02-02 332

　　Objective: To investigate the effects of various administration methods on the function of hematopoietic stem cells in mice.

　　Methods: C57 mice were intraperitoneally injected with busulfan and divided into high-dose group (40 mg/kg) and low-dose group (20 mg/kg). The low-dose group was given once, and the high-dose group was given for 2 days, daily 1 20 mg/kg. Detect mouse peripheral blood and bone marrow cell counts, hematopoietic stem cells, hematopoietic progenitor cells, and long-term hematopoietic stem cell ratio 15 and 30 days after administration, evaluate the proliferation ability of mouse hematopoietic progenitor cells through CFU-GM test, and perform blood flow analysis Count, I will do it. Selection of long-term hematopoietic stem cells The function of hematopoietic stem cells cultured in vitro was tested through single-cell colony experiments.

　　Result: Both the high-dose and low-dose groups of busulfan can reduce the number of white blood cells and platelets in the peripheral blood of C57 mice, and reduce the proportion of hematopoietic stem progenitor cells and long-term hematopoietic stem cells. Reduce CFU-GM and single cell colony forming ability. The injury effect of the high-dose group was even worse than that of the low-dose group. There was no significant difference between the mouse body weight and the control group.

　　Conclusion: High-dose busulfan can induce hematopoietic stem cell damage 15 days after administration, which provides a model and basis for studying the damage mechanism and damage protection of hematopoietic stem cells.