动物造模,大鼠Pig-a基因突变 z-bio 2021-02-02 313

　　Objective: To study the effect of different doses of N-ethyl-N-nitrosourea (ENU) and cyclophosphamide (CP) on the deletion rate of ankyrin CD59 on the peripheral red blood cell surface of SD rats, and to optimize the Pig-a gene mutation test Detection method.

　　Method: The SD rats were divided into 4 groups according to body weight and peripheral blood RBCCD59, namely vehicle control group, CP40 mg/kg group, ENU10 mg/kg group, ENU40 mg/kg group, each group had 6 abdominal cavities. For internal exposure only, the vehicle control group was injected with PBS solution. Weighing and blood sampling were performed before and after 7, 14, 21, 28, 42 and 56 days after exposure, and the incidence of peripheral blood RBCCD59- was detected by flow cytometry.

　　 Results: Compared with the vehicle control group, there was no significant difference in body weight and weight gain between the ENU 10 mg/kg and ENU 40 mg/kg groups at each time point. Weight loss and weight gain in the CP40 mg/kg group at each time point (P\u003c0.05)? CP40 mg/kg group was administered on 28-42 and 56 days, ENU 10 mg/kg group was administered on 42 and 56 days, ENU 40 mg/kg group was administered in peripheral blood RBCCD59-after administration. The incidence increased from 7 days to 14, 21, 28, 42 and 56 days (P\u003c0.05), and there was a dose-response relationship.

　　 Conclusion: In the Pig-a gene mutation test, ENU has a better effect on increasing the incidence of RBCCD59 in peripheral blood of rats than CP, with a dose of 40 mg/kg greater than 10 mg/kg.