动物造模,大鼠背根神经节 z-bio 2021-11-04 308

　　Purpose: To explore a new method for quickly, conveniently and effectively extracting rat dorsal root ganglia (DRG)---searching and extracting DRG from the direction of nerve walking.

　　 Method: Use traditional and new methods of opening the intervertebral foramen to extract DRG, and compare the time taken by the two extraction methods with the number of complete DRGs extracted. Results: The number of intact dorsal root ganglia from L3 to 5 segments extracted from each rat by conventional methods was (3.08±1.31) minutes, and the average time per DRG was (5.58±1.21)) minutes; The new method for the number of complete dorsal roots The root ganglia of L3 to 5 segments extracted from each rat are (4.29±1.08), and the average time per DRG is (1.69±0.91) minutes, which is similar to the conventional method. There is a significant difference than. (P\u003c0.05).

　　 Conclusion: A new method of searching and extracting the nerve gait direction of DRG can extract the complete rat dorsal root ganglion more effectively. This provides more information about dorsal root ganglion cell culture and morphological studies. experiment. Reliable organization materials.