动物造模,2型糖尿病大鼠 z-bio 2021-11-04 306

　　Objective: To study the effect of antioxidant N-acetylcysteine (NAC) on oxidative stress and FoxO1 activity in the liver of type 2 diabetic rats?

　　 Method: Did you randomly divide 24 male SD rats into a normal control group (C)? -Treatment group (D) and NAC treatment group (D + AC) (n = 8)? Has type 2 diabetic rats been established through a low-dose intraperitoneal injection and a high-fat diet streptozotocin (STZ) model? Group D+AC group NAC 1.5 g/kg gavage, C group and D group were given the same amount of normal saline. After eight weeks, is the automatic biochemical analyzer plasma triacylglycerol (TG)? Free fatty acids (FFA)? Aspartate aminotransferase was detected. (AST)? Alanine aminotransferase (ALT) level, is the kit for liver superoxide dismutase (SOD)? Catalase (CAT)? Detection of glutathione peroxidase (GSH-Px) adenosine triphosphate (ATP) and malondialdehyde peroxide (MDA) content in plasma and liver tissue); Western blot analysis of caspase-3 in liver tissue Analyze the expression level of FoxO1 protein in the cytoplasm. Does this indirectly reflect the activity level of FoxO1?

　　Result: Compared with group C in plasma TG? FFA? AST? ALT? MDA and MDA content in liver tissue? Caspase-3 expression level? The activity of FoxO1 in group D rats was significantly increased, but the liver tissue SOD? ATP levels and GSH-Px activity are significantly reduced; AC treatment can inhibit the changes of the above indicators in diabetic rats after 8 weeks, is the difference statistically significant?

　　 Conclusion: The antioxidant NAC can alleviate the liver dysfunction related to diabetes. Its mechanism may be related to the inhibition of oxidative stress, the reduction of mitochondrial dysfunction in diabetes and the over-activation of FoxO1?