动物造模,椎间盘脱出大鼠 z-bio 2021-12-06 534

　　Objective: To observe the effects of electroacupuncture combined with methylprednisolone and electroacupuncture alone or methylprednisolone alone on the motor function of the intervertebral disc and neurons in rats with disc herniation. Is this reasonable and provides a basis for clinical application? : 112 SD rats in the random sham operation group (sham)? Model (model)? Before electroacupuncture combined with methylprednisolone (EA + MP)? (Divided into electro-acupuncture group (EA) and methylprednisolone group (MP)), a self-made silicone gasket compression method was used to make a disc herniation model, and each group was treated separately to obtain the BBB limb motor function score . Second or collected spinal cord tissue has Nestle staining. Results: 1) The BBB scores showed an upward trend after treatment in each group. The EA + MP group was significantly higher than the EA MP group on the 3rd to 7th day after hind limb compression (P0.05) 2) Nissle staining was compared with the model group. Each treatment group had less damage to nerve cells and a larger proportion of Nissl bodies. Among them, is the EA + MP group better than the other two groups? (3) The incidence of side effects, 30% in MP group, 4.16% in EA + MP group, and EA0 group; mortality, 20% in MP group, 0% in EA + MP group and EA group?

　　 Conclusion: Three treatment methods can increase limbs to varying degrees. Within 24 hours (beyond the time range of the action of methylprednisolone), the number of cadavers of rats with herniated discs can maintain neuromorphology and improve limb motor function. Among them, EA + MP is the most effective, which can significantly reduce the side effects and mortality of MP treatment.