动物造模 z-bio 2021-02-03 348

　　Objective: To explore the interventional effect and mechanism of low molecular weight heparin calcium on mouse embryo implantation.

　　Methods: A mouse pregnancy model was constructed, and LMWHC was injected intraperitoneally to intervene to observe the effect of LMWHC on mouse embryo implantation. Mouse trophoblast cells were isolated and cultured, LMWHC intervened with different concentrations of cells, cell invasion was detected by Transwell chamber, and ELISA was used. Western blot and RT-PCR were used to detect the protein and mRNA level of matrix metalloproteinases-MMP-2 and tissue inhibitor of metalloproteinase-TIMP-2 in the cell supernatant.

　　Results: After intraperitoneal injection of LMWHC in pregnant mice, the number of embryos implanted in D6 and D9 mice was significantly increased (P<0.05); 1×102 IU/L, 1×103 IU/L and 1×104 IU/L were used After 24h of LMWHC intervention with trophoblast cells, the number of trophoblast cells invaded was (84.83±14.15), (162.48±25.49), (98.73±12.77), and (65.27±13.92) without LMWHC intervention. ) Comparison, the difference was significant (P<0.05), the expression level of MMP-2 in the trophoblast cell supernatant was higher than that in the cells without LMWHC intervention (P<0.05), and the TIMP in the trophoblast cell supernatant -2 expression level is lower than that of cells without LMWHC intervention (P<0.05).

　　Conclusion: LMWHC can promote mouse embryo implantation, and its mechanism may be related to the enhancement of trophoblast cell invasion by LMWHC by affecting the expression of MMP-2 and TIMP-2.