动物造模,卒中后抑郁模型 z-bio 2022-03-17 377

　　Objective: How to study the effect of fluoxetine on the behavior and hippocampal neurogenesis of post-stroke depression (PSD) rats?

　　 Method: 32 adult male rats were randomly divided into sham operation group, stroke group, PSD group and fluoxetine. Did Tin Group use the middle cerebral artery embolization method to create a rat model of ischemic stroke, and combine chronic unpredictable mild stimulation with orphan methods to create a PSD rat model? During the baseline period and postoperative days 7, 14, 21, and 28, the rats were weighed on the same day as the sucrose preference test and the unboxing test to assess the behavioral changes of the rats. A mandatory swimming test was added on the 35th day. At the end of the study, immunohistochemistry was used to detect neuronal regeneration in the hippocampal dentate rotation. On the 28th and 35th days after the onset of stress, there were significant differences in the weight of the rats, the percentage of water consumed by sucrose, the out-of-box test, and the horizontal and vertical movement scores between the PSD and stroke groups (P\u003c0.05) ). , The fluoxetine group was more significant. Is the difference in the PSD group also statistically significant (P\u003c0.05)? Compared with the stroke group, did the proportion of rats in the PSD group increase significantly in the immobile time forced swimming test? Has the swimming and climbing time been greatly reduced (P\u003c0.01)? After fluoxetine intervention, is the immobility time of rats significantly shorter than that of the PSD group? Does the swimming time increase significantly (P\u003c0.01)? Immunohistochemistry showed that compared with the stroke group, the number of new neurons in the hippocampal dentate gyrus of the PSD group was significantly reduced (P\u003c0.01), while the number of new neurons in the hippocampus of rats was significantly reduced after fluoxetine intervention. Compared with the PSD group (P\u003c0.01)?

　　 Conclusion: Can fluoxetine effectively improve depression in PSD rats? Does hippocampal neuron regeneration play an important role in this process?