动物造模,感染免疫应答 z-bio 2021-12-24 340

　　Purpose: After highly pathogenic avian influenza infects mice, will over-expressed inflammatory factors or inflammatory factor storms be detected in the lungs? Is high inflammation more common in older people infected? This article studies the impact of inflammatory storms in the lungs of the elderly, and the impact of the host's immune response to infection?

　　Method: Have you established an old mouse infection model by administering H9N2 influenza virus to older mice (18-24 months)? The control group is adult mice (6-8 circles) infected with H9N2. )? Quantitative analysis of the expression of inflammatory factors in the lungs and peripheral blood of H9N2 infected elderly mice, and during the acute phase of infection, the collected lung tissues of the mice were sliced and digested into single cell suspensions. These lung tissue sections are used for immunohistochemical in situ quantitative analysis of immunocytochemical distribution; are T cells sorted, stained and counted from cell suspension?

　　Result: Compared with adult mice, older mice were infected with H9N2 virus. Have you lost a lot of weight and your survival rate is only 50%? However, compared with the lung tissue of adult mice after infection, almost no inflammatory factors were detected in the lungs of old mice. The chemokine MCP-1 is abnormally highly expressed. The peak value (4000 pg/mL or higher) is reached on the second day after infection, which is 100 to 1000 times that of adult control mice. The responsiveness of lung macrophages in elderly mice infected with H9N2 virus was lower than that of adult control groups. , Lung neutrophils have weak migration ability and stay in the lungs 3-7 days after infection? In addition, on the 7th day after H9N2 infects elderly mice, the number of lung CD8 + T cells is significantly reduced. Are the number and proportion of CD4 + T cells normal?

　　 Conclusion: After H9N2 virus is infected with H9N2 virus in aging mice, is there an inflammatory factor storm in the lung tissue? The cell type response of anti-infection immunity is similar to that of adult mice. There is no significant difference, but is the migration of immune cells reduced and the acquired cell-mediated immunity participating in the response greatly reduced?