动物造模,结肠癌血管生成抑制作用 z-bio 2021-02-03 303

　　Objective: To explore the inhibitory effect of Shenchang Capsule combined with 5-fluorouracil (5-fluorouracil, 5-FU) on colon cancer growth and angiogenesis and its possible mechanism?

　　 Method: Establish 50 BALB/c mouse LoVo colon cancer models, randomly divide them into 5 groups according to different treatment methods, and divide them into model groups? 5-FU group? Shenyi capsule low-dose group? Shenyi Capsule high-dose group? Shenyi capsule + 5-FU group? The test records mouse tumor volume, tumor microvessel density (MVD), immune changes, and tumor Gli1 detected by western blot? VEGF protein expression?

　　Result: 1) Compared with the model group, is the tumor volume of mice in each treatment group significantly suppressed? Shenyi Capsule high-dose group? Does the Shenyi Capsule + 5-Fu group show the highest tumor inhibition rate, and does this inhibition become more obvious as the administration time increases? (2) The organ quality and indicators of the mice in the 5-FU immunized group were lower than those in the model group, but the other treatment groups improved to varying degrees. The quality of thymus, thymus index, spleen quality and spleen index improved the most in the Shenyi capsule + 5-FU group. obviously. , Significantly better than all other treatment groups (P0.05)? High-dose Shenyi capsules? The colon cancer metastasis rate of each organ in the Shenyi capsule + 5-FU group was significantly reduced (P0.05)? Shenyi Capsule high-dose group? In the Shenyi capsule + 5-FU group, is Gli1 protein? VEGF protein expression is significantly reduced (P\u003c0.05)?

　　 Conclusion: Shenyi Capsules can significantly inhibit the growth and angiogenesis of colon cancer cells. Combining this inhibitory effect with 5-FU makes it more obvious. Can the expression of VEGF related to the Hedgeog-Glin1 signaling pathway become its main target?