动物造模,小鼠肺纤维化 z-bio 2021-11-06 340

　　Purpose: Three different methods were used to intubate C57BL/6J mice to induce pulmonary fibrosis, using lumbar needle tracheal intubation, indwelling needle tracheal intubation and tracheostomy to study tracheal intubation. Tube model. Will the indwelling needle tracheal intubation model and tracheostomy model affect mice?

　　Method: 120 C57BL/6J Do you randomly divide the mice into blank groups? Lung puncture needle tracheal intubation model group? In the indwelling needle tracheal intubation model group and the tracheostomy model group, have you used different methods to inject bleomycin into the trachea and observe the overall condition (weight change and survival rate) of the mice? Histopathological changes (HE and Masson staining) and hydroxyproline levels?

　　Result: Lung puncture needle tracheal intubation model group? 28 days after modeling, the weight of the mice in the indwelling needle tracheal intubation model group was higher than that in the tracheostomy model group, the difference was statistically significant (P\u003c0.01?P\u003c0.001); the incidence of mouse survival Except for the blank group, the tracheotomy model group was the lowest, and the indwelling needle tracheal intubation model group was the highest. There were statistical differences between the blank group and the tracheotomy model. There was no statistical difference between the other two groups (P\u003c0.05); is the degree of alveolar pneumonia and pulmonary fibrosis in the tracheotomy model group? Lung puncture needle tracheal intubation model group and indwelling needle tracheal intubation model group, the heaviest 21 days after modeling; the distribution of lesions is uniform in tracheotomy model group, lumbar spine needle tracheal intubation model group and indwelling needle tracheal intubation model group The distribution is uneven, and the lung tissue around the trachea is heavier; in the lumbar puncture tracheal intubation model group and the tracheostomy model, the hydroxyproline level 28 days after modeling, this group was significantly higher than the blank group (P \u003c0).0.01? P\u003c0.001)?

　　 Conclusion: Combining multiple factors, is lung puncture and tracheal intubation modeling the best of the three modeling methods?