动物造模,2型糖尿病 z-bio 2022-12-28 577

　　Objective: How to study the effect of curcumin on glucose transport and phosphatidylinositol kinase 3 (PI3K)/protein kinase B (Akt) signaling pathway in adipocytes of type 2 diabetes (T2DM) rats? Methods: 90 SD rats were randomly divided into normal group, model group (high-fat diet), low-dose curcumin group (50 mg/kg), medium-dose curcumin group (150 mg/kg), and high-dose curcumin group (250 mg/kg). mg/kg). kg)), Lodgeitazone group (1.35 mg/kg), 15 rats in each group? Take it immediately after taking it? 2 weeks of management? After the administration for eight weeks, the rats were weighed, administered for eight weeks, and fasted for 12 hours for blood sampling. Fasting blood glucose was detected in each of them (FBG fasting insulin (FINS) expression? Insulin resistance index (HOMA-), IR calculation) , Triglycerides (TG)? Low/high-density lipoprotein cholesterol (LDC-C/HDL-C)? Total cholesterol (TC) expression? The positive glucose clamp test can measure the perfusion rate of glucose. Fluorescence immunoassay detects the translocation of glucose transporter 4 (GluT4) in the cell membrane of fat. Real-time fluorescence quantitative PCR (qRT-PCR) detects GluT4 and Western blot in fat cells. GluT4 inside and outside fat cells? P-PI3K? Is insulin receiving body substrate 2 (Irs2)? Expression of p-Akt.

　　Result: Is the model group administered immediately than the normal group? 2 weeks after the administration, the body weight increased and 8 weeks weight loss; compared with the model group, the curcumin treatment group and rosiglitazone group lost weight compared with the model group (P\u003c0.05). Model group FBG? FINS? HOMA-IR? HDC-L? LDC-L? TC? TG increased, while the glucose infusion rate decreased in a dose-dependent manner. , GluT4 mRNA increases, and GluT4? Irs2? P-PI3K/PI3K? PAkt/Akt protein expression increased. Significantly. Decrease; the above difference is statistically significant (P\u003c0.05)? Curcumin treatment group? Rosiglitazone group FBG? FINS? HOMA-IR? HDC-L? LDC-L? TC? TG level is significantly reduced; glucose infusion rate is significantly increased; GluT4 mRNA is reduced; GluT4? Irs2? P-PI3K/PI3K? The expression of PAkt/Akt protein increased significantly. The above differences are statistically significant (P\u003c0.05), and the three differences are also statistically significant in the curcumin treatment group (P\u003c0.05)?

　　Conclusion: Curcumin may increase insulin sensitivity in T2DM rats and I2DM rats. It activates the PI3K/Akt signaling pathway. This pathway promotes GluT4 membrane transport in adipocytes and improves glucose absorption in adipocytes and blood buds. It reduces glucose metabolism Balance.