动物造模 z-bio 2021-02-04 365

　　Purpose: How to observe the effect of combined intake time of different doses of fluoride and aluminum on longitudinal bone growth and bone metabolism in rats?

　　Method: 48 8-week-old clean SD rats weighing 170-190 g were randomly divided into normal control groups, which were low-fluoride aluminum and high-fluoride aluminum groups, respectively, 45d and 90d groups. Do you perform bone morphology analysis on the proximal tibial growth plate and posterior pituitary cancellous bone?

　　Results: Compared with the normal group, the growth plate of the high aluminum fluoride group was thickened. The chondrocytes in the 45d group were arranged neatly and had no abnormal morphology, but the cartilage cells in the 90d group were densely retained; secondary to the low fluoride aluminum (45d and 90d) group around metabolism Trabecular bone calcification? Bone formation rate? In the 90d group, osteoblasts around and around bone resorption increased; in the high aluminum fluoride 45d group, did the above bone metabolism index increase, and in the 90d group, did the above bone metabolism index decrease?

　　Conclusion: Short-term exposure to high aluminum fluoride will stimulate cartilage growth and long-term inhibit longitudinal bone growth? Short-term exposure to low-fluoride aluminum will only increase the secondary bone formation of the trabecula. Long-term exposure to low-fluoride aluminum and short-term exposure to high-fluoride aluminum will stimulate the bone formation and bones of the trabecula, increase absorption and produce high-fluoride aluminum, which can inhibit bone formation and absorption in the long term. ?