动物造模,哮喘小鼠 z-bio 2021-02-04 304

　　Objective: To observe the effect of Fujiu Ointment Affiliated to Liaoning University of Traditional Chinese Medicine on serum interleukin 4 (IL-4) and interferon-γ (IFN-γ) in asthmatic mice. Explore the expression of protein E (immunoglobulin E, IGE) cytokines, nerve growth factor (NGF) and tyrosine kinase A (tropomyosin receptor kinase A, TrKA) in lung tissues and the therapeutic mechanism of asthma?

　　Methods: Only 50 rats were randomly selected from A normal control group, B asthma model group, C dexamethasone group, D Fujiko paste high-dose group, E Fujiko paste low-dose group, 5 groups, Obbomin (OVA) Adult BALB/c mice. ). ) Intraperitoneal injection to sensitize, ultrasonic spray to stimulate inhalation, establish an asthma model? ELISA method detects serum IL-4, total IGE, OVA specific IGE and IFN-γ cytokine expression, HE staining and Masson staining to observe pathological changes, immunohistochemistry to observe NGF expression? TrKA protein in lung tissue?

　　Result: Compared with the normal group, the NGF of the model group? TrkA and IL-4? Total IGE? The expression of OVA-specific IGE increased significantly (P0.05)

　　Conclusion: Vojiu cream can down-regulate the expression of NGF