动物造模 z-bio 2021-10-27 279

　　Objective: To study the effect of curcumin pretreatment on changes in blood and tissue neutrophil counts in hot swimming rats in a hot and dry desert environment?

　　 Method: 160 SPF male SD rats are selected and divided into random groups. For the four curcumin dose groups, each group was given the same dose for one week. The rats were placed in a special environmental artificial experimental cabin in the northwest, and the climate mode of arid and hot desert environment was set to 0 minutes each time. ? 50 minutes? 100 minutes? At 150 minutes, each group randomly removes 10 animals from anesthesia and determines the number of neutrophils in the blood; at 150 minutes, each group takes circumflex branches, liver, kidney and lung tissues to measure the rats. Measure the number of rats, observe under the HE staining microscope, and record the number of infiltrating neutrophils and tissue damage?

　　Result: The blood neutrophils of each group showed an upward trend from 0 to 50 minutes. The blood neutrophils in each group tended to decrease from 50 minutes to 150 minutes. The ileum, liver, kidneys and lungs remain neutral for 150 minutes. As the pretreatment concentration of curcumin increases, the number of grains infiltration tends to decrease. The transfer of neutrophils to the high-concentration group was significantly lower than that of the low-concentration group and the saline group (P\u003c0.01), and reached heat stroke within 150 minutes. At that time, Pearson correlation analysis showed blood neutrophil count and tissue neutrophil infiltration. Is there a significant negative correlation?

　　 Conclusion: Under conditions of continuous exposure to dry heat, the number of blood neutrophils in rats tends to increase first and then gradually decrease. With the intensification of tissue damage, the number of tissue infiltrating neutrophils increases. Is the decrease of blood neutrophils closely related to the increase of tissue neutrophils? Curcumin may inhibit heat stroke. Will it reduce blood neutrophil depletion, tissue neutrophil infiltration and tissue damage in mice?