动物造模,大鼠心肌miRNA-30a表达 z-bio 2021-02-04 348

　　Objective: To study the effects of acute exercise on rat myocardial miRNA-30a and the expression of autophagy-related genes, and to explore the regulatory mechanism of miRNA-30a on myocardial autophagy activity in different recovery periods after exercise.

　　Methods: SD rats were randomly divided into a quiet control group (Con), a 0h post-exercise group (E0), a 3h post-exercise group (E3) and a 12h post-exercise group (E12). The materials were taken at different time points after the acute exercise. Use real-time Fluorescence quantitative polymerase chain reaction (PCR) was used to detect ACE mRNA, miRNA-30a, Beclin-1 mRNA and LC3 mRNA expression in left ventricular muscle.

　　Results: Compared with the quiet control group, the expression of ACE mRNA and miRNA-30a in the left ventricular muscle of the 0h group after exercise was significantly increased (P<0.05), and the expression of Beclin-1mRNA and LC3 mRNA was also significantly increased (P<0.05). Recovered to a quiet level 3h after exercise.

　　Conclusion: Acute exercise can significantly increase the expression of myocardial ACE mRNA, miRNA30a, Beclin-1 mRNA and LC3 mRNA. This transient increase may be caused by exercise stress.