动物造模,小鼠睾丸损伤 z-bio 2021-02-04 303

　　Objective: To study the protective effect of triamcinolone on testicular injury in heat-stressed mice?

　　 Method: 120 male ICR mice were randomly divided into normal control group, model group, triamcinolone drug intervention group (25 mg/kg) and 50 mg/. Kg, 100 mg/kg), 24 mice per group? Except for the normal control group, each group of mice was given the corresponding oral medication for 1 week, and the lower abdomen of each group was heated at a constant temperature of 43°C. After 15 minutes of pressure treatment in a water bath, the dose of triamcinolone corresponding to the drug intervention group was taken orally for 14 days, the normal control group and the model group were given normal saline for 14 days, and the materials were ingested 1. After heat stimulation treatment Days 7, 10 and 14. Do you want to calculate the genital index, sperm count and HE staining to observe and compare the changes in testicular tissue structure?

　　Result: Compared with the normal control group, the genital coefficient and the number of epididymal sperm of the model group were significantly reduced (P\u003c0.05), and histopathology had obvious damage to the testis of the mice. Show it? Compared with the model group, each dose in the drug intervention group may increase the organ coefficient of mouse reproductive organs and epididymal sperm counts (P\u003c0.05); histopathology, whether the drug intervention group shows heat stress Can the testicular damage caused by mice be significantly improved?

　　 Conclusion: Does trigonelline have a specific protective effect on the testis of mice due to heat stress?