动物造模 z-bio 2021-02-04 332

　　Objective: How to evaluate the effect of high-fat diet-induced obesity on the ovarian function of female rats and its mechanism?

　　Method: randomly divide 80 female Wistar rats into a high-fat diet (HFD) group (n = 40) and a normal diet (control group) group (n = 40)? After eating for 60 days, assess the estrus cycle of each group of rats, the number of cells and apoptosis in each stage of rat follicular development, and measure the serum progesterone and estradiol levels of each group, and then measure the male and female. Rat 1:1. Did you put it in a cage at a ratio of 2 for 3 weeks?

　　Result: HDF induced obesity and hyperglycemia in rats, but did not affect serum triglycerides. Rat cholesterol and C-reactive protein levels? Does obesity also change ovarian function, prolong the estrus interval of HFD rats, reduce serum estradiol levels and increase the number of closed follicles of pyloric antlers? In addition, did the rats in the HFD group also develop follicular cysts? Is the pregnancy time of HFD rats later than that of control rats?

　　 Conclusion: Does obesity induce follicular cysts in rats, interfere with the ovaries and cause delayed pregnancy?