动物造模,肺组织炎症 z-bio 2021-11-16 432

　　Objective: To study the effect of chronic PM2.5 exposure on mouse pneumonia and NLRP3 inflammasome activity, and to provide a new target for the prevention and treatment of lung injury caused by PM2.5?

　　Method: Male C57BL/6J mice were exposed to different doses of PM2.5 via tracheal infusion, and the normal saline dose of control mice was 2,10 mg/(kg?bw). The mice were injected 20 consecutive times and blood was collected. Every 3 days. And lung tissue? Blood counts were performed on three groups of mice; immunofluorescence staining was used to detect the level of macrophages in lung tissue; the level of interleukin (IL)-1β, IL-18 and caspase-1 activity; whether quantitative PCR was used for detection in real time The expression level of NLRP3 inflammasome-related mRNA in lung tissue?

　　Results: Two doses of PM2.5 exposure can significantly reduce the proportion of monocytes (P\u003c0.01) and increase the proportion of neutrophils (P\u003c0.01) leading to the development of pneumonia; lung tissue caspase Increase of -1 activity (P\u003c0.01) and NLRP3 and ASC mRNA expression (P\u003c0.01)? Compared with the control group, did the levels of IL-1β and IL-18 in the lung tissue of the two dose groups increase significantly (P\u003c0.01)?

　　Conclusion: Will long-term exposure to PM2.5 activate the NLRP3 inflammasome in the lung tissue and cause pneumonia?