动物造模,2型糖尿病 z-bio 2021-10-28 320

　　Objective: To observe the damage effect of busulfan (busulfan) on the hematopoietic system function of type 2 diabetes model KK/upj-Ay/J (KKAy) mice, and compare it with the control C57BL/6J (B6) mice.

　　Methods: Male KKAy mice were randomly divided into a busulfan administration group and a solvent control group. The former was intraperitoneally injected with busulfan at a dose of 40 mg/kg, the latter was injected with the same dose of 5% DMSO, and B6 mice were treated the same. Grouping. Weigh 15 days after administration, calculate immune organ coefficient, detect peripheral blood count and classification of mice, and detect hematopoietic progenitor cell (HPC), hematopoietic stem cell (hematopoieticstemcell, HSC) and long-term hematopoiesis in bone marrow by flow cytometry The proportion of stem cells (longterm-hematopoieticstemcell, LT-HSC). Colony-forming unit-granulocyte and macrophage (CFU-GM) assay was used to evaluate the function of mouse hematopoietic progenitor cells.

　　Results: 15 days after Busulfan administration, the body weight, white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), blood platelet (PLT), lymphocyte (LYM) of KKAy mice ), neutrophil (NEU), the proportion of HPC, HSC and LT-HSC in the bone marrow were significantly reduced, the number of CFU-GM was significantly reduced, and the proportion of spleen and thymocytes did not change significantly. After Busulfan administration, the WBC, RBC, HGB and LYM of KKAy mice were significantly lower than those of B6 mice, but the ratio of PLT and HSC was significantly higher than that of B6 mice. The reduction of PLT and HSC of KKAy mice was significantly lower than that of B6 mice, while the reduction of HPC was significantly higher than that of B6 mice.

　　Conclusion: 40mg/kg busulfan administration can damage the hematopoietic system function of mice. After Busulfan administration, WBC, RBC, HGB and LYM were more severely damaged in KKAy mice, but PLT and HSC were less damaged and more tolerated, while the tolerance of hematopoietic progenitor cells was lower than that of B6 mice.