How to prepare an animal model of hemolytic anemia?
动物模型,溶血性贫血
z-bo
2020-08-03
678
Hemolytic anemia (HA) is a common type of anemia. It causes red blood cells to survive for a short time and is destroyed for some reason. It exceeds the compensation capacity of the bone marrow. It is a common hematopoietic disease.
[Modeling mechanism] Inject a certain amount of acetophenazine (APH) into animals. APH is a powerful oxidant, especially slows down red blood cells and gradually oxidizes them, thereby interfering with red blood cells. Glucose 6-phosphate dehydrogenase promotes hemoglobin denaturation to form the body of He, directly destroys red blood cell membrane proteins and lipids, dissolves the membrane and degrades red blood cells, thereby causing hemolytic anemia.
[Modeling method]
1. The mice were injected subcutaneously with 0.2 g/kg, 0.1 g/kg, and 0.2 g/kg APH saline on day 1, 4 and 7, and the model was successfully established on day 9. Other management modes may also lead to this model.
2. The rats were injected subcutaneously with 0.16 g/kg and 0.08 g/kg APH saline on the 1st and 4th day, and the model was successfully created on the 8th day. Or, the two doses are 0.2 g/kg, and the model is successfully created on the 10th day.
3. In order to establish a hemolytic anemia model, 2% APH saline was injected subcutaneously or intramuscularly into the rabbit 2-3 times at a dose of 0.1 g/kg.
Observation Index: General behavior, observation of general growth status, such as hair, bleeding points, death, etc., to determine routine peripheral blood parameters, such as red blood cell count, white blood cell count, platelet count, hemoglobin content, etc. Changes in blood cytochemical indicators, such as neutrophil alkaline phosphatase, acid phosphatase, adenylate triphosphate, succinate dehydrogenase, glucose-6-phosphatase and reticulocyte counts, Hestia, bone marrow Check other indicators.
[Model Features] After injection of APH, the animals in the experimental group had reduced activity, dull hair, reduced food intake, weight loss, fatigue, skin discomfort and paleness. .. The hemoglobin and red blood cells in the peripheral blood gradually decrease, and the reticulocyte, hay body and white blood cell counts increase significantly. Neutrophil alkaline phosphatase, acid phosphatase, adenylate triphosphate, succinate dehydrogenase, glucose 6 phosphatase and bone marrow are all different in the degree of abnormal changes.
[Model Evaluation and Application] The method of injecting strong oxidant APH can create an animal model of hemolytic anemia that meets the clinical characteristics of APH dose. It should be explored through preliminary experiments. I can. This method has a short modeling period and is easy to operate. The symptoms of model animals and the biochemical changes of surrounding blood and blood cells are basically the same as those of human hemolytic anemia. A simple model is provided.
