失血性贫血,动物模型 z-bo 2020-08-03 585

　　Anemia after acute hemorrhage is anemia caused by rapid heavy bleeding, while anemia after chronic hemorrhage is small cell anemia caused by long-term moderate bleeding.

　　[Modeling mechanism] The blood volume and blood cells of animals are reduced by artificial blood, which leads to short-term anemia characteristics in animals, which is a model of hemorrhagic anemia.

　　[Modeling method] Experimental animals are mice, rats, rabbits and dogs. The specific method is as follows. Six to eight drops (approximately 0.5 ml) of retroorbital venous plexus bleeding in mice form a model of chronic hemorrhagic anemia five times every other day. Cutting the tail and bleeding 0.5 ml every day for 7 consecutive days can cause anemia.

　　2. Take the rat's tail and draw 1.5-2 ml of blood every other day for 5 consecutive times to form chronic hemorrhagic anemia.

　　3, rabbits or dogs collect 1/3 of the total blood volume from the vein or artery of the animal once a day, and then repeat it several times to create an animal model of hemorrhagic anemia. [Characteristics of the model] After blood collection, the number of blood cells and hemoglobin in the animal can be significantly reduced and detected. If the degree of anemia needs to be increased, the number of blood collection and the number of blood collection can be appropriately increased. [Model Evaluation and Application] Acute hemorrhagic anemia is caused by the discharge of a large amount of blood in a short time. Chronic hemorrhagic anemia is also caused by repeated bleeding. This method is easy to operate and has clear indicators, but it is difficult to control blood loss between samples. This model is used to study the causes of common hemorrhagic anemia and the pharmacodynamics of new drugs.