动物造模 z-bio 2021-02-19 282

　　Purpose: When cultivating sterile rats, artificial milk given after birth is very important for cultivating sterile rats. Deeply hydrolyzed lactose-free protein milk powder is easily absorbed by infants and reduces various indigestion and allergic reactions. Next, adding this milk powder to the milk used by sterile rats has a positive effect on the cultivation of sterile rats. Detect the biochemical indexes of sterile rats in normal feeding.

　　Method: Use clean SD rats to determine the delivery time. After removing the uterus, the rat pups were peeled off with an isolator and artificially breast-fed until 22 days after weaning. Record body weight and survival rate. In the formula used, milk powder without deep hydrolyzed protein lactose was added to the experimental group, and milk powder at a regular price was added to the control group. At the 8th week, the blood biochemical indexes of the normally cultured sterile rats were tested.

　　Result: In the first 14 days of artificial feeding, there was no difference in body weight and survival rate between the experimental group and the control group. From the 14th day, the weight and survival rate of the experimental group were higher than those of the control group. Compared with the control group, the survival rate of the experimental group to day 22 was significantly improved (37.18% vs. 17.77%), and the body weight on day 22 was also significantly higher than that of the control group (9.96±0.49) vs. (13.36) ±0.59 ). The blood biochemical indicators of sterile rats showed a decrease in AST (aspartate aminotransferase) and an increase in GLU (glucose).

　　 Conclusion: In the process of raising sterile rat pups, lactose-free milk powder with deep hydrolyzed protein can be added as an important component of formula milk. This can effectively increase weight and reduce the mortality of young rats. The blood biochemical indexes of female infertile rats have changed significantly.