动物造模,高脂血症易感 z-bio 2022-01-10 342

　　Objective: To observe the etiology and pathological characteristics of hyperlipidemia (WSHc) rats with spontaneous hindlimb paralysis, study the etiology in advance, and explore its application and scientific research value.

　　Method: In the WSHc rat population cultivated in the center, 8 rats with spontaneous hindlimb paralysis and 8 non-paralysis rats of the same age were fed a normal diet and a high-fat diet. Observe your sensitivity to hyperlipidemia. Magnetic resonance imaging and histopathology were used to observe the central nervous system diseases in different parts of the hindlimb paralyzed rats. TUNEL immunohistochemical method was used to observe the level of apoptosis; to detect the expression system of caspase-1 and IL-1β genes in the nerves of different parts of the central nervous system, And by Western Blot method using RT-PCR to detect protein levels.

　　Result: Both male and female WSHc rats with paralyzed hind limbs can suffer from this disease, and the sensitivity to high-fat diet is not significantly different from that of non-paralyzed WSHc rats. Magnetic resonance imaging showed no obvious brain and cerebellum. Compared with WSHc rats without hindlimb paralysis, in WSHc rats with hindlimb paralysis, a large number of inflammatory cell infiltration and TUNEL expression were observed in the posterior spinal cord, and caspase-1 and IL expression were positive. The posterior 1β gene in the spinal cord increased significantly (P\u003c0.05, P\u003c0.01), and protein expression also increased significantly (P\u003c0.05).

　　 Conclusion: Spontaneous hindlimb paralysis in hyperlipidemia-sensitive WSHc rats is a progressive disease, which occurs in the middle and back of the spinal cord. The pathological features are inflammatory cell infiltration and neuronal degeneration and apoptosis.