动物造模,单纯疱疹病毒Ⅱ型 z-bio 2021-12-21 321

　　Objective: To compare the pathological changes of BALB/c mice infected with herpes simplex virus type II (HSV-2) by different routes.

　　Method: Three different attack methods were used to infect BALB/c mouse HSV-2 through the vagina, nasal cavity and cornea. The nerves were detected by observing the clinical symptoms of the mice and the pathological changes of the attack site and nerve tissue. Organizational viral load.

　　 Results: The mice in the nasal cavity group had no obvious symptoms, but the survival rate at the end of the observation period was only 27.27%. The mice in the vagina group showed varying degrees of vulvitis and weight loss. The ratio is 0; mice in the cornea group showed varying degrees of keratitis, and the survival rate was still 63.64% at the end of the observation period. The pathological changes only showed obvious pathological changes in the brain tissue of the nasal cavity group; the vagina and cornea groups showed obvious pathological changes in the attack site, while the mice in the vagina group showed obvious pathological changes in the brain and spinal cord. The results of the neural tissue viral load test showed that compared with the control group, the viral load of HSV-2 in the brain and spinal cord of the three experimental groups increased significantly and increased with the increase of infection time.

　　 Conclusion: BALB/c mice infected with HSV-2 in three different ways can cause different degrees of pathological changes in mice. This study provides a theoretical basis for the etiology of neurological diseases caused by HSV-2 infection.