动物造模,5型腺病毒 z-bio 2021-02-20 274

　　Purpose: To use four methods to detect the distribution of adenovirus type 5 (Ad5) in C57BL6J after infection.

　　Method: 112C57BL/6J divide mice into 4 groups. 100 μL of 1×109 pfu/mL Ad5 solution was injected into each mouse by nasal infusion, gastrointestinal feeding, tail vein and intraperitoneal injection on the first 1, 2, 3, and 4 days after infection. Fluorescence quantification of 2 male and female mice, lymph nodes, testes, epidermis, uterus, ovary, spleen, kidney, liver, lung, heart, brain, blood and bone marrow tissues were collected for PCR at the expense of 5d, 6d and 7d groups Detection. Results: Ad5 was detected in the lung and spinal cord tissue of the nasal drip group, and Ad5 in the lung tissue was further reduced 4 days after infection; Ad5 was not detected in all tissues of the liver and spleen in the gastrointestinal feeding group, and was not detected in the tail vein Ad5 was detected in the injection group, and the decline rate was relatively slow. Ad5 was detected in the spinal cord, spleen, epididymis and testis of the intraperitoneal injection group. The content of Ad5 in the spinal cord of the intraperitoneal injection group was about 10 times that of the nasal fluid group. The content of Ad5 in the spleen was similar to that of the tail vein injection group. Postnasal drip and testicular Ad5 are only 1% of the content detected in other positive tissues. About 10%.

　　 Conclusion: Ad5 lung infection experiment can be performed every 3 days. Intravenous tail can be used for liver infection experiments. Intravenous or intraperitoneal injection can be used for spleen infection experiments.