动物造模 z-bio 2021-11-06 340

　　OBJECTIVE: To obtain the full-length coding sequence of the conjugative adhesion molecule A (AJAM-A), analyze its molecular characteristics, and use JAM as the receptor for Leovirus that invades the primary lung cells of the conjugative adhesion molecule. 

　　Method: Extract total RNA from healthy tree ferret tissues, and use reverse transcription PCR and cDNA endoderm cloning technology to obtain JAM-4 gene. UniproUGENE uses the most possible method of MEGA 6.0 software to analyze the encoded amino acids and phylogenetic tree; real-time immunofluorescence PCR is used to detect the expression and distribution of JAM-A in 25 tissues and blood of the tree. Primary lung epithelial cells are treated by receptor-specific antibodies on lung epithelial cells, and then immunofluorescence is used to observe the viral antigen on Leo. The impact of orphan virus infection. By obtaining the full-length 9622bp sequence of JAM-A gene cDNA, phylogenetic analysis showed that the genetic relationship between JAM-4 gene and human is closer than that of rodents. JAM-A molecules have high levels of peri-tree tissue expression in the respiratory and gastrointestinal tracts. The specific antibody acts on the cells and significantly reduces the integrated immunofluorescence absorbance value of the virus antigen (P\u003c0.0001).

　　 Conclusion: The gene sequence of JAM-A has been cloned and analyzed for the first time, confirming that JAM-A molecule is the main receptor for reovirus invading trees, and Shangshu can be used as a new animal model of reoviridae.